Urea-based synthetic urine and method of manufacturing same

ABSTRACT

A synthetic urine solution and method of its manufacture are disclosed. The solution includes water having a pH between about 3 and about 10. The solution further includes creatinine, a means for removing bacteria from the solution so as to control or eliminate sepsis of the urine solution, preferably through the use of a biocide, and a urea-based compound. The solution exhibits a specific gravity of from 1.005 g/cm 3  to 1.025 g/cm 3 . Additional compounds may also be included to further enhance the aesthetics or apparent authenticity of the synthetic urine.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application claims priority to U.S. Provisional Application No.61/819,775 entitled “UREA-BASED SYNTHETIC URINE AND METHOD OFMANUFACTURING SAME,” filed on May 6, 2013, which is hereby incorporatedby reference in its entirety.

BACKGROUND OF THE INVENTION

This invention relates to a composition and method of manufacturingurea-based synthetic urine. More specifically, the synthetic urineincludes biocides to allow for increased shelf stability.

An example of a method of manufacturing synthetic urine may be seen byreference to U.S. Pat. No. 7,192,776, the disclosure of which is herebyincorporated by reference in its entirety.

The kidneys remove unwanted substances circulating in the blood by wayof producing urine, which is excreted from the body. Consequently,diverse waste substances and other substances unwanted by the body findtheir way into urine for subsequent removal from the body. Urinalysis isthe testing of the composition and amounts of waste substances in urine,and provides a tremendously powerful diagnostic tool for the medicalprofession. In particular, many of these substances are indicative ofcertain medical conditions or other substances which have beenmetabolized by a person's kidneys.

Using current urinalysis techniques, unwanted substances in a urinesample can mask existing medical conditions, while still some others canmasquerade as non-existent medical conditions. In each instance, theseunwanted substances undermine the usefulness of urinalysis as a medicaldiagnostic tool. Some of the unwanted substances that find their wayinto a urine sample are drugs (both legal and illegal) and metabolitesthereof, along with other chemical residues or contaminants that may bepresent or otherwise contacted during the handling procedures. Thesesubstances can disturb the sensitive tests, making the actual state ofthe body difficult or impossible to determine.

For example, urea compounds, uric acid, insulin levels,para-aminohippuric acid, phenol sulfonphthalein, phosphate,arylsulfatase-A, lysosome, urine amylase, total urine estrogens,specific estrogens, progestins, aldosterone, catecholamines,5-hydroxyindoleacetic acid, cortisol, homovanillic acid, human chorionicgonadotrophin, creatine, bilirubin, hemoglobin, hydroxyproline, melanin,porphorins, total protein, acid mucopolysaccharide, copper, glucoseoxidase and urine ketone can all influence the results of most standardurinalysis testing methods in unintended or unpredictable ways.

Essentially, these testing methods include a variety of immunoassays orassays by other techniques, such as isolation followed by gas or liquidchromatography followed by mass spectrometry. These tests makeurinalysis a powerful diagnostic tool for identifying a whole range ofconditions. For example, substance abuse and other indicia of disease orbodily state can easily be detected by urinalysis. However, in order toaccurately establish standards of comparison for such tests, reliableurine samples are needed which are entirely free from any of theaforementioned substances. Thus, the development of a suitable,synthetic urine substitute would improve testing methods by providingresearchers, potential urine donors and testing technicians with anaccurate baseline reading for “clean” urine samples to compare againstother suspect samples.

To illustrate, a method for detecting this compound is described in U.S.Pat. No. 5,036,014, issued to Elsohly et al., where various deuteratedcannabinoids are synthesized to help determine the quantitative amountof tetrahydrocannabinol in a urine sample. One method in particularinvolves spiking a clean urine sample with known amount of deuteratedtetrahydrocannabinol and analyzing the resultant sample with gaschromatography/mass spectrometry in order to establish set standards ofcomparison. However, a failure to possess a truly clean sample couldsubstantially influence and negatively affect the results of thesemethods.

Another example of the problems created by interfering chemicals inurine is exemplified by the case of ibuprofen. Ibuprofen is aprostaglandin synthetase inhibitor that may be taken in large doses torelive pain and inflammation characteristic of arthritis. When a patienttaking these massive doses is subjected to urinalysis, it may mask otherdrugs being taken by the donor, or may even be mistaken fortetrahydrocannabinol (a metabolite which many testing techniciansclassify as being indicative of marijuana use).

Any misidentification of controlled substance use/abuse, personalinformation (pregnancy, use of cigarettes, etc.) or any of the numerousmedical conditions that can be determined using urinalysis can havedevastating personal consequences for the urine donor. Thus, somecompanies sell inexpensive home testing kits in order to provide somelevel or reassurance to potential urine donors whether they may havesuch a misidentification. However, given the potential liability for amisidentified or positive test, many lay persons feel intimidated bytesting procedures, and these persons would welcome the ability toutilize a known sample, free from unwanted or unknown substances, forthe sake of comparison.

In response to the need for a reliable source of relatively inexpensive,“clean” urine samples which are free from any unwanted or unknownsubstances, numerous attempts to formulate synthetic urine have beenmade. For example, U.S. Pat. No. 6,306,422 to Batich et al. (table 3,col. 16, line 50 et seq.), U.S. Pat. No. 5,328,954 to Sarangapani (table1, col. 9, line 29 et seq.), U.S. Pat. No. 5,489,281 to Watanabe et al.(col. 12, example 6) and U.S. Pat. No. 4,146,644 to Griffith et al.(table 1, col. 10). However, none of these references appears to addressa simple composition which can be manufactured in an inexpensive mannerthat contains urea or can be supplemented with urea, and hasshelf-stability from use of biocides.

Additionally, all of these references require the use of creatinine orother compounds which can be consumed by bacteria present in the sample.Accordingly, all of these samples will undergo sepsis unless they areimmediately used, thereby making these compounds unattractive candidatesfor mass production and/or consumer sales.

SUMMARY OF THE INVENTION

It is an object of this invention to provide a reliable source ofurea-based synthetic urine, along with a method for its manufacture,which is free from any and all unwanted or unknown substances.

It is a further object of this invention to provide a urea-basedsynthetic urine, along with a method for its manufacture, which iscapable of retaining its viability and utility for extended periods oftime, for example with use of biocides.

Still further uses for such a synthetic urine can and will be devised bya prospective user based upon his or her own personal disposition,interests and privacy concerns.

Accordingly, a composition of synthetic urine is claimed. Thiscomposition includes water, having a pH between about 3 and about 10with creatinine and biocide. A urea compound is provided in conjunctionwith the synthetic urine solution. The urea may be dissolved at the timeof manufacture and/or prior to testing of the synthetic urine. Thecomposition further includes any compound which dissolves anddissociates in a water solution in a manner which insures that thespecific gravity of the resulting solution mixture is between about1.005 g/cm³ and about 1.025 g/cm³.

The addition of a urea compound, either during manufacture or at a latertime, is another element of the invention, and those skilled in the artwill readily identify appropriate specific types of biocide oxiders,organics or in situ agents, along with a host of carbonates, halidesalts, hydroxide salts and other chemicals which could serve as idealionic compounds within the meaning of the invention.

A method for manufacturing synthetic urine involves providing water,dissolving creatinine and biocide in the water, adjusting the resultingsolution's specific gravity to be between about 1.005 g/cm³ and about1.025 g/cm³ and, if necessary, adjusting the pH level of the solution. Aurea compound is provided in conjunction with this synthetic urinesolution. In another aspect, a method for manufacturing synthetic urineinvolves providing water, dissolving creatinine and a biocide in thewater, adjusting the resulting solution's specific gravity to be betweenabout 1.005 g/cm³ and about 1.025 g/cm³ and, if necessary, adjusting thepH level of the solution, and adding the urea compound at a subsequenttime, for example, prior to testing the synthetic urine. Alternatively,the urea compound is dissolved in the synthetic urine solution duringmanufacturing.

In another aspect, the method contemplates providing water with a pHbetween about 3 and about 10, mixing creatinine and at least onedissociating ionic compound to adjust the specific gravity of theresulting solution to be between about 1.005 g/cm³ and about 1.025 g/cm³and removing bacteria from the solution so as to avoid sepsis of thecreatinine. A urea compound is provided in conjunction with thissynthetic urine solution for subsequent addition to the solution.Alternatively, the urea compound is dissolved in the synthetic urinesolution.

In each of these embodiments the same types of biocides, urea compounds,and ionic compounds can be used as were identified in the compositionembodiments above.

DETAILED DESCRIPTION

While human urine may at varying times reflect a wide range of chemicalcompounds, current urinalysis relies upon observation of four basictraits: pH level, specific gravity, the presence of creatinine, and thepresence of urea. Consequently, it was discovered that an effective, yetcost efficient, synthetic urine solution having a final specific gravitybetween about 1.005 g/cm³ and about 1.025 g/cm³ needed only to containfour basic components: water with a pH between about 3 and about 10;creatinine; some means for controlling or eliminating the unwantedsepsis of the creatinine; and some means for controlling the amount ofurea. For example, sepsis control/elimination is most readilyaccomplished through the use of a biocide.

Examining each of these four traits separately, the need for awater-based solution should be apparent. However, it is significant tonote that human urine can display a wide range in terms of pH variation,anywhere from about 3 to about 10. This variation can be attributed toany number of factors regarding regional water quality, metabolicidiosyncrasies displayed by each individual and the like. Thus, thewater supplied for the composition and method may need to have its pHadjusted accordingly. Significantly, while use of distilled, deionizedwater will produce the most reliable synthetic urine solutions in termsof elimination of unwanted substances, the invention may be practicedwith equal efficacy using distilled water, deionized water or evenregular tap water (drawn from any fresh water source having anappropriately low specific gravity, as discussed below). Unlessspecifically noted to the contrary, use of the term “water” throughoutthis specification and appended claims is intended to embrace thebroadest array of appropriate water sources available.

Urea, also referred to as carbamide, is produced by the body aftermetabolizing protein. A urea compound is produced when the liver breaksdown protein or amino acids, and ammonia; the kidneys then transfer theurea from the blood to the urine. A person can excrete 30 grams of ureaa day, mostly through urine, but a small amount is also secreted inperspiration. Urea can also be naturally produced by other mammals, suchas certain reptiles and amphibians. Synthetic versions of the chemicalcompound can be created in liquid or solid form. Unless specificallynoted to the contrary, use of the term “urea compound” throughout thisspecification and appended claims is intended to embrace the broadestarray of appropriate urea compound sources available.

The final synthetic urine solution must also have a specific gravitybetween about 1.005 g/cm³ and about 1.025 g/cm³. Insofar as specificgravity is a measure of relative ionic content of a solution, it shouldbe apparent to those familiar with body chemistry or kidney functioningthat certain ions and compounds will be commonly found in human urine,especially those commonly encountered in food and water sources (forexample, sodium, potassium, chloride, etc.). In contrast, other elementswill be inherently unwise choices at anything beyond a trace level (forexample Lanthanoid and Actinoid series ions). The precise amount of theparticular compound or compounds selected to adjust the specific gravitywill depend directly on the concentration of the particular compound orcompounds (if in solution), the molecular weight of its constituents,water temperature, relative volume of water solvent being used and othersimilar factors. With respect to the water used to manufacture thesynthetic urine of the present invention, it is anticipated thatsignificant increases will need to be made to the specific gravity, asdistilled, deionized water has a specific gravity of 1.000 g/cm³ and tapwater, while likely to vary by region, has a specific gravity around1.003 g/cm³.

In terms of the best compounds to utilize in adjusting the specificgravity, the single most important trait is that the compound mustdissociate when dissolved in water. Additionally, it is preferred tofind an inexpensive, widely available compound so as to minimizeproduction costs. To that end, it is believed that carbonate salts,halide salts, hydroxide salts and certain bromides will have particularapplicability. By way of illustration rather than limitation, thesesalts might include sodium bicarbonate, sodium, potassium, magnesium orcalcium chlorides; sodium, potassium, or calcium hydroxides; and othersimilarly inexpensive and widely available salts.

Creatinine is a protein created in connection with muscular activity. Assuch, medical science recognizes creatinine as an important constituentin the human bloodstream and, to the extent that the kidneys cleanse andpurify the bloodstream, in the waste stream expelled from the kidneys inthe form of urine. Significantly, because creatinine is a protein, it isthe subject of sepsis and decomposition. Thus, creatinine serves as anexcellent indicator in urinalysis because it is indicative of humanorigin and, by virtue of its septic disposition, creatinine alsoprovides a natural measure to determine whether or not a sample was, infact, recently produced.

In order to insure stable creatinine levels in synthetic urine, it istherefore essential to remove or control the presence of sepsis-causingbacteria. However, whatever method of control is applied must also notinterfere with the processes underpinning most urinalysis techniques.Thus, the use of an appropriate biocide is absolutely critical to theefficacy of the synthetic urine. To the extent that human urine issterile when excreted (under normal body conditions), the use of biociderepresents a distinct departure from previous approaches to themanufacture of synthetic urine which relied solely on mimicking thecompounds in actual urine without any regard for the long-term shelflife of the synthetic solution. Moreover, it further demonstrates theneed to select a biocide which is biologically active, yet does notinterfere overtly with the chemistry of the synthetic urine solutionitself (either through its chemical signature or by virtue of anabnormally large amount being detectable in the solution).

A biocide can be generically defined as a substance used to control oreliminate microbial populations in a sample. Generic examples ofbiocides that have particular applicability when used in connection withthe present disclosure include, but are not limited to the following:oxidizing biocides, organic biocides, and a somewhat more generalizedcategory referred to as in situ agents. Non-limiting examples will bediscussed briefly below, although it should be understood that biocidesare a term of art, known to those familiar with water chemistryprocesses.

Oxidizing biocides are generally self explanatory. This class includesany biologically effective agent which relies upon an oxidation process,including but not limited to various peroxides, hypochlorites, bromidesand super oxides. Organic biocides encompass an expansive list ofproteins and cyclical compounds known to those skilled in the art. Insitu agents can be chemical compounds or actual physical processesdesigned to kill bacteria in a manner which is either self-generating oreffective enough to prevent future degradation of the urine. Genericexamples of such in situ agents include ozone, chlorine dioxide (orother dioxides), and ultraviolet radiation or irradiation processesfollowed by hermetic sealing of the sample.

Specific examples of various biocides contemplated above include: BHAP(such as 2-Bromo-4-hydroxyacetophenone, an organo-bromine group);Bronopols (such as 2-Bromo-2-nitropropane-1,3 diol, an organo-brominegroup); Carbamates (such as a mix of sodium dimethyldithiocarbamate(DIBAM) and disodium ethylene bisdithiocarbamate (NIBAM), or singleproduct, such as potassium n-hydroxymethyl-n-methyldithiocarbamate, anorgano-sulfur group); Chlorothioether (such as 2,2Dihydroxy-5,5-dichlorodiphenyl monosulfide, a chlorinated phenolicthioether); DBNPA (such as 2-2-Dibromo-3-nitrilopropionamide, anorgano-bromine group); DTEA, DTEA II (such as 2-(Decylthio)ethanamine,an alkylthioamine group); Guanides (including Guanidine and Biguanides)(such as dodecylguanidine hydrochloride and acetate, alsopolyhexamethylene biguanide hydrochloride, and tetradecylguanidine, allaliphatic guanadines); Glutaraldehydes (such as Pentane-1,5-dial., analdehyde group); Isothiazolines (such as Alkyl isothiazolin-3-ones, anorgano-sulfur group); MBT (such as Methylene bis(thiocyanate), anorgano-sulfur group); Polyquats (such as broad-spectrum, cationicpolymers of low molecular weight); Quats (ADBACs) (such asAlkyldimethylbenzylammonium chloride (also known as alkylbenzyldimethylammonium chloride or benzalkonium chloride), a quaternary ammoniumcompound group); Sulfones (such as Bis(trichloromethyl)sulfone, anorgano-sulfur group); TBTO (such as Bis(tributyltin)oxide, an organo-tingroup); TBZ (Tertbuthylazine) (such as2-(tert-butylamino)-4-chloro-6-(ethylamino)-s-triazine, a Triazinegroup); TCCBN (such as Tetrachloro-2,4,6-cyano-3-benzonitrile, TCCBNfunctions similarly to the chlorophenols); TCMTB (such as2(thiocyanomethylthio)benzothiazole); Thiones (such asTetrahydro-3,5,dimethyl-2H-1,3,5-thiadiazine-2-thione, an organo-sulfurgroup); THPS (TKHPS) (such as Tetrakish(hydroxymethyl)phosphoniumsulfate, an alkyl phosphonium group); and TTPC (such asTributyltetradecylphosphonium chloride, an alkylphosphonium group).Additionally, with respect to more commonly understood items, such asperoxides, hypochlorites and the like, it should be understood that thisspecification encompasses all forms of such compounds (for example,hydrogen peroxide, sodium peroxide, sodium hypochlorite, potassiumhypochlorite, etc.). Other examples of biocides may exist and variousexemplary embodiments are encompassed within this specification.

Notably, as embraced by this disclosure, oxidizing biocides, andhypochlorite in particular, should not be confused with the agents thatare employed to oxidize metabolites in urine samples. Such metaboliteoxidizers are often referred to as “adulterants” within the urinalysisindustry. Adulterants are substances deliberately added to actual urinesamples to chemically alter the metabolites indicative of certainconditions so as to render these metabolites undetectable by standardurinalysis techniques.

Even though some substances like hypochlorite may possess utility asboth a biocide and as an adulterant, the intended use of that substance(as either a biocide or a metabolite oxidizer) will substantiallyinfluence the conditions, concentration and manner in which thesubstance is provided. In particular, use as a biocide requires smallerconcentrations and little to no regard for when the biocide is addedduring the manufacturing process. To illustrate, an oxidizing biocidesuch as sodium hypochlorite can be added in amounts as small as 1 mL per3.8 L of water. Similar concentrations of other oxidizing biocides willhave equal efficacy, as recognized by those skilled in the art.

In contrast, use of hypochlorite as an adulterant as taught, inter alia,in U.S. Pat. No. 6,861,262 must occur at higher concentrations and in aspecific manner so as to oxidize certain metabolites or compounds. Thus,hypochlorite (and other oxidizing biocides) found in the presentsolution prevents the unwanted growth of bacteria. Moreover, to theextent that adulterants are often added to actual urine samples, thecomposition of the resulting mixture is substantially more complex, interms of the variety of chemical species present, than the simplifiedcomposition of the present invention.

Another aspect of the present urine solution relates to the addition ofurea in some form to the synthetic urine sample. Urea or carbamide is anorganic compound with the chemical formula CO(NH₂)₂. Urea serves animportant role in the metabolism of nitrogen-containing compounds byanimals and is the main nitrogen-containing substance in the urine ofmammals. Urea compounds can be produced from inorganic startingmaterials. Examples of urea compounds include, but are not limited to,carbamide peroxide, allantoin, and hydantoin.

Urea is increasingly being tested for in urinalysis techniques. Itspresence within synthetic urine could add an additional level of realismfor some applications. For example, the addition of urea to syntheticurine may serve as a scent producing material to make the syntheticurine appear more natural. Notably, to the extent that urea is provided,it will need to be considered in the calculations of the amount of ionicdissociating compounds required to adjust the specific gravity and/or pHto the desired levels. Urea compounds can be dissolved in the watersolution or synthetic urine solution. As an optional addition, a sugarmay be dissolved with the urea.

Providing a shelf stable synthetic urine with a biocide having a killfactor for a specified period, such as 1-2 years, will keep the productsterile. Although 1-2 years is specified, this is a non-limiting exampleof a kill factor time period. Other desired time periods may be optimal.The urea compound may be added to the water or synthetic urinepreferably just prior to testing of the urine. In an embodiment, theurea compound is added to the water or synthetic urine duringmanufacture. A shelf stable synthetic urine with biocide can be placedin the US and other markets around the world. Similarly, the syntheticurine is formulated for use by various customers in these varyinglocations.

Other functionally inconsequential additives or steps may also beincluded without departing from the principles of this invention. Whilethese additives and steps expressly cover all foreseeable equivalents ofthe elements recited above, additional variations are possible. Forexample, it is possible to include a coloring agent and or olfactorysubstance to enhance the aesthetics or apparent authenticity of thesynthetic urine produced according to this invention.

The foregoing detailed description has been given for clearness ofunderstanding only and no unnecessary limitations should be understoodtherefrom as some modifications will be obvious to those skilled in theart without departing from the scope and spirit of the appended claims.

We claim:
 1. A synthetic urine solution comprising: water having a pHbetween about 3 and about 10; creatinine and a biocide, said creatinineand biocide dissolved within said water to form a solution exhibiting aspecific gravity and said creatinine and biocide selected in relativeconcentrations to minimize sepsis; at least one dissociated ioniccompound also dissolved within said solution to adjust the specificgravity of the solution to between 1.005 g/cm³ and 1.025 g/cm³; whereinsaid biocide is selected from at least one of2-bromo-4-hydroxyacetophenone, bronopols, carbamates, chlorothioethers,2-2-Dibromo-3-nitrilopropionamide, 2-(Decylthio)ethanamine,glutaraldehydes, isothiazolines, Methylene bis(thiocyanate), polyquats,Alkyldimethylbenzylammonium chloride, sulfones, bis(tributyltin) oxide,tertbuthylazines, Tetrachloro-2, 4,6-cyano-3-benzonitrile,2(thiocyanomethylthio)benzothiazole, thiones,Tetrakish(hydroxymethyl)phosphonium sulfate,Tributyltetradecylphosphonium chloride, peroxides, hypochlorites, andsuper oxides; and at least one urea compound provided in conjunctionwith the synthetic urine solution.
 2. The synthetic urine solution ofclaim 1, wherein the at least one urea compound is either dissolvedwithin said solution or provided separately to be dissolved within saidsolution at a subsequent time.
 3. The synthetic urine solution of claim1, wherein the at least one urea compound is selected from at least oneof carbamide peroxide, allantoin, and hydantoin.
 4. The synthetic urinesolution of claim 1, wherein said at least one ionic compound isselected from at least one of carbonate salts, halide salts, hydroxidesalts, and bromides.
 5. A method of manufacturing a synthetic urinesolution comprising: providing water; dissolving creatinine and biocideinto said water to form a solution exhibiting a specific gravity level,said creatinine and biocide being selected in relative concentrations tominimize sepsis, wherein said biocide is selected from at least one of2-bromo-4-hydroxyacetophenone, bronopols, carbamates, chlorothioethers,2-2-Dibromo-3-nitrilopropionamide, 2-(Decylthio)ethanamine,glutaraldehydes, isothiazolines, Methylene bis(thiocyanate), polyquats,Alkyldimethylbenzylammonium chloride, sulfones, Bis (tributyltin) oxide,tertbuthylazines, Tetrachloro-2,4,6-cyano-3-benzonitrile, 2(thiocyanomethylthio)benzothiazole, thiones,Tetrakish(hydroxymethyl)phosphonium sulfate,Tributyltetradecylphosphonium chloride, peroxides, hypochlorites, andsuper oxides; adjusting said specific gravity level of said solution tobetween 1.005 g/cm³ and 1.025 g/cm³; and providing at least one ureacompound in conjunction with the synthetic urine solution.
 6. Thesynthetic urine solution of claim 5, wherein the at least one ureacompound is either dissolved within said solution or provided separatelyto be dissolved within said solution at a subsequent time.
 7. Thesynthetic urine solution of claim 5, wherein the at least one ureacompound is selected from at least one of carbamide peroxide, allantoin,and hydantoin.
 8. The method of claim 5 further comprising sealing saidsynthetic urine solution within a container so as to further minimizesepsis of said synthetic urine solution.
 9. The method of claim 5,further comprising the step of adjusting the pH level of the solutionbetween about 3 and about
 10. 10. A method of manufacturing a syntheticurine solution comprising: providing water having a pH between about 3and about 10; dissolving creatinine and at least one dissociating ioniccompound in the water to form a solution exhibiting a specific gravity,said creatinine and at least one dissociating ionic compound selected inrelative concentrations to adjust said specific gravity to between 1.005g/cm³ and 1.025 g/cm³; adding a biocide into said solution, said biocideis selected from at least one of 2-bromo-4-hydroxyacetophenone,bronopols, carbamates, chlorothioethers,2-2-Dibromo-3-nitrilopropionamide, 2-(Decylthio)ethanamine,glutaraldehydes, isothiazolines, Methylene bis(thiocyanate), polyquats,Alkyldimethylbenzylamnionium chloride, sulfones, Bis(tributyltin) oxide,tertbuthylazines, Tetrachloro-2,4,6-cyano-3-benzonitrile, 2(thiocyanomethylthio)benzothiazole, thiones,Tetrakish(hydroxymethyl)phosphonium sulfate,Tributyltetradecylphosphonium chloride, peroxides, hypochlorites, andsuper oxides; removing bacteria from said solution; and providing atleast one urea compound in conjunction with the synthetic urinesolution.
 11. The synthetic urine solution of claim 10, wherein the atleast one urea compound is either dissolved within said solution orprovided separately to be dissolved within said solution at a subsequenttime.
 12. The synthetic urine solution of claim 10, wherein the at leastone urea compound is selected from at least one of carbamide peroxide,allantoin, and hydantoin.
 13. The method of claim 10, further comprisingthe step of sealing said synthetic urine solution within a container.14. The method of claim 11 wherein the step of dissolving creatinine andat least one dissociating ionic compound also includes dissolving the atleast one urea compound in the water, said at least one urea compoundselected in a concentration relative to that of said creatinine and atleast one dissociating ionic compound so as to maintain the specificgravity of the solution between 1.005 g/cm³ and 1.025 g/cm³.
 15. Themethod of claim 14, further comprising the step of sealing saidsynthetic urine solution within a container.
 16. The method of claim 10,wherein the bacteria are removed from said solution using an in situagent selected from at least one of ozone, dioxides, ultravioletradiation, and irradiation processes followed by hermetic sealing of thesolution.